| date | lecture topic | recommended reading |
|---|---|---|
| January 20 | Course introduction; Nucleic acid structure |
Chapter 1 |
| January 22 | DNA manipulation: denaturation and hybridization | Chapter 4 |
| January 25 | Amplifying DNA: PCR | Chapter 4 |
| January 27 | Amplifying DNA: PCR & its many uses | Chapter 4 |
| January 29 | Gene identification & DNA libraries | Chapter 4 |
| February 1 | Amplifying DNA: restriction enzymes, vectors & cell-based cloning | Chapter 4 |
| February 3 | Amplifying DNA: restriction enzymes, vectors & cell-based cloning | Chapter 4 |
| February 5 | Article 1: Buck and Axel (1991) | Reading posted on Blackboard. Complete the exercises on this web site before reading the article: |
| February 8 | Positional cloning | supplemental reading posted on Blackboard |
| February 10 | Positional cloning | supplemental reading posted on Blackboard |
| February 12 | Expression of cloned DNA | Chapter 6, pp. 132-137, 147-149 |
| February 15 | Expression of cloned DNA |
Chapter 6, pp. 132-137, 147-149 |
| February 17 | Purification of expressed proteins |
|
| February 19 | Genome structure | Chapter 5, pp. 115-121 |
| February 22 | Genome structure: transposons | Chapter 7, pp. 159-171 |
| February 24 | Genome structure: transposons | Chapter 7, pp. 159-171 |
| February 26 | Analyzing genomes Midterm exam distributed |
Chapter 12 |
| March 1 | Forensic DNA analysis | Chapter 16 |
| March 3 | Forensic DNA analysis Midterm exam due in class |
Chapter 16 |
| March 5 | Article 2: Coble et al. (2009) | Reading posted on Blackboard. |
| March 8-12 | SPRING BREAK | |
| March 15 | Prokaryotic transcription: RNA polymerase and promoters | Chapter 3, pp. 53-59 |
| March 17 | Prokaryotic transcription: transcription initiation | Chapter 3, pp. 53-59 |
| March 19 | Article 3: Johnson, Moran and Losick (1983) | Reading posted on Blackboard. |
| March 22 | Eukaryotic transcription: promoters |
Supplemental reading posted on Blackboard. |
| March 24 | Eukaryotic transcription: transcription factors and enhancers | Chapter 3, pp. 62-70; supplemental reading posted on Blackboard. |
| March 26 | Article 4: Kutach and Kadonaga (2000) | Reading posted on Blackboard. |
| March 29 | RNA processing | Chapter 5, pp. 105-114 |
| March 31 | RNA processing | Chapter 5, pp. 105-114 |
| April 2 | RNA processing | Chapter 5, pp. 105-114 |
| April 5 | Epigenetics | Chapter 8 |
| April 7 | Epigenetics | Chapter 8 |
| April 9 | Article 5: Fraga (2005) | Reading posted on Blackboard. |
| April 12 | Prokaryotic transcription termination |
Chapter 3, pp. 60-61; supplemental reading posted on Blackboard |
| April 14 | Riboswitches | Chapter 2, 48-50; supplemental reading posted on Blackboard |
| April 16 | Article 7: Mandal et al., (2003) | Reading posted on Blackboard. |
| April 19 | RNAi | Chapter 9 |
| April 21 | RNAi | Chapter 9 |
| April 23 | Article 8: Bagga et al. (2005) | |
| April 26 | Enhancers & enhancer blockers | supplemental reading posted on Blackboard |
| April 28 | Enhancers & enhancer blockers | supplemental reading posted on Blackboard |
| April 30 | Course wrap-up |