Home
Experiment Schedule
Course Materials
Lectures
Links & Resources |
Schedule of Experiments
|
 |
instructor |
date |
experiment title (click for more) |
techniques |
| SB & SW |
Sept. 5/6 |
Reading Scientific Papers / Reading Structures |
materials posted on Moodle |
| |
|
|
|
| SB |
Sept. 12/13 |
Two Ligand Syntheses: |
organic synthesis |
| |
|
(1) Pteridinyl Phenanthroline and (2) Porphyrin |
|
| SB |
Sept. 19/20 |
Ru(bpy)2(pter-phen)2+ Complex Synthesis |
coordination synthesis, ESI-MS, 1H NMR |
| |
|
|
|
| SB |
Sept. 25/26 |
Fe(porphyrin)Cl Synthesis
Characterization of Metal Complexes. Part I |
2-D COESY NMR, absorption spectroscopy |
| |
|
|
|
| SW |
Oct. 3/4 |
Biochemical Techniques. I. Lysozyme |
careful pipetting, UV/vis, |
| |
|
|
determination of protein concentration by 2 methods |
| SW |
Oct. 10/11 |
Biochemical Techniques. II Myoglobin Denaturation |
UV/vis, determination of thermodynamic parameters |
| |
|
|
|
| SW |
Oct. 24/25 |
Biochemical Techniques. III Lactate Dehydrogenase |
UV/vis, kinetics and catalysis |
| |
|
|
|
| SB |
Oct. 31/NOV. 1 |
Characterization of Metal Complexes. Part I |
CV, UV/vis |
| |
|
|
|
| SB |
Nov. 7/8 |
Characterization of Metal Complexes. Part II |
CV, magnetic susceptibilty |
| |
|
|
|
| SW |
Nov. 14/15 |
Metal-Biomacromolecular Interactions. |
DNA Melting, |
| |
|
|
Photocleavage monitored by gel electrophoresis |
| SB |
Nov. 28 |
Discussion of Fe-porphyrin results |
|
| SW |
Nov. 29 |
Metal-Biomacromolecular Interactions |
Fluorescence titration |
| |
|
|
|
| SW |
DEC. 5/6 |
Metal-Biomacromolecular Interactions |
|
| |
|
|
|
| SB & SW |
DEC. 12/13 |
grand finale! |
|
| |
|
|
|
| |
|
|
|
| |
| |
|
class activities |
documents
|
| Wed.- Sept. 12 |
1) describe two bioinorganic projects |
|
| |
2) introduce ligands and synthetic paths |
|
| |
3) go over groups, synthesis chart and procedures |
|
| |
4) in lab, set up and start phen-pteridine ligand reactions (except for Group 2 making L-allox) |
|
assignment |
calculate masses of reagents for phen-pteridine ligand reaction using information in chart |
|
| |
|
|
| Thurs.- Sept. 13 |
1) do porphyrin synthesis |
|
| |
2) isolate phen-pteridine ligand |
|
| |
(Group 2: make L-allox) |
|
|
|
|
| Wed.- Sept. 19 |
|
|
assignment |
1) prepare to share your thoughts on the two synthetic procedures you were given last week |
|
| |
|
|
| Wed.- Sept. 26 |
|
|
assignment |
1) have filtered your Ru complex |
|
|
|
|
| Thurs.- Sept. 27 |
- do porphyrin synthesis |
|
| |
- isolate Ru complex (if you haven't already done so) |
|
| |
- observe process for obtaining 2D COSY NMR |
|
| |
|
|
| Wed.- Oct.3 |
|
|
assignment |
1) Draft 1 of the Experimental section of the Ru manuscript, with an outline for Introduction |
|
| |
|
|
| Wed.- Oct.31 |
|
|
assignment |
1) Draft 2 which includes: Introduction, revised Experimental, Results including syntheses and NMR data analysis |
|
|
|
|
| Thurs.- Nov. 1 |
- CV experiment of Ru-phen-pteridine complexes |
|
| |
|
|
| Thurs.- Nov. 8 |
- magnetic measurements on Fe-porphyrin, with and without imidazole |
|
| |
- CV experiment on Fe-porphyrin, with and without imidazole |
|
| |
|
|
| Wed.- Nov. 28 |
- in class presentation and discussion of results of measurements on Fe-porphyrin series |
|
| |
|
|
| |
|
|