Bryn Mawr College K-16 Collaborations in Science and Math Education Homepage
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Left Picture by Blythe Hoyle
Activities and Schedule for Sept. 28, 2007
Morning: Preparing, Sampling, Identification, Mapping, Deploying Leaf Packs
Afternoon: Identify, Discuss, Photograph Benthic Macro-invertebrate
Benthic sampling protocolAquatic insects are adapted to avoiding predation in their environment. As a result, if we want to catch aquatic insects, we need to minimize disturbance to their micro-habitat prior to sampling. This means we students should approach stream sampling sites from downstream and avoid tromping in sampling areas. Sampling streams with a D-net requires two people working together. Transferring samples from net into zip-lock bags also works best with two people.
Protocol --Stream sampling 1. Approach the sampling site from downstream, minimizing splashing. 2. Take any depth measurement you might need at sample site. 3. Label ziplock bag with site identifying information. 4. Person 1: Place D-net on stream bed with opening facing upstream and net firmly pressing into stream bottom. Tilt net slightly back to permit access of net opening by person 2. 5. Person 2: Disturb rocks in front of the net to dislodge stream insects and encourage flow of dislodged insects into the net. Steam insects have evolved to hanging on to substrate in fast moving water. For small streams like Mill Creek the best way to do this is: • Use your hand or a rock to disturb the stream bottom in front of the net opening. • Swirl water towards the net opening • Pick up the surface layer of rocks in square area in front of the net opening for a distance upstream approx. equivalent to width of net. • One by one scrub the surface of each rock with your hands to dislodge clinging insect larvae. • Set aside each rock after scrubbing. • Disturb sediment in lower rock layer, swirl water/bugs into net. • Scoop net upwards so as it prevent insects from escaping. • Invert contents of net into ziplock bag (preferably over a bucket or sieve/tray). Check net to make sure there are no stragglers. • Add enough water to cover contents of sample (so that they survive the trip back to the lab)
Protocol --Pond sampling 1. Minimize splashing/disturbing the sampling site. 2. Take any depth measurement you might need at sample site. 3. Label ziplock bag with site identifying information. 4 Use D-net to scoop across the pond bottom, removing an area of sediment equivalent to width of net base squared. The majority of larvae we are interested in capturing will occur in the top layer of sediment so try to capture the top inch of sediment as you scoop. 5. Move the net to buckets of pond water and rinse contents by dipping the net bag into the water. MAKE SURE TO KEEP NET OPENING ABOVE WATER WHILE YOU DO THIS. 6. Invert contents of net into ziplock bag (preferably over a bucket or sieve/tray). Check the net to make sure there are no stragglers. 7. If your gallon ziplock is less than 1/3 full of sediment you should scoop more. More than ½ full is probably overdoing it. 8. Add enough water to cover contents of sample (so that they survive the trip back to the lab) Note: Both stream and pond samples ziplocks should be placed in buckets, transported back to lab and kept cool, preferably refrigerated until after-lunch viewing.
The activities, schedule and protocol were developed by Wil Franklin (Senior Lab Instructor at BMC)
DefinitionsEcosystem: An ecosystem is a natural system consisting of all plants, animals and microorganisms (biotic factors) in an area functioning together with all the non-living physical (abiotic) factors of the environment. (wikipedia encyclopedia) Ecology: Is the scientific study of the distribution and abundance of living organisms and how the distribution and abundance are affected by interactions between the organisms and their environment. The environment of an organism includes both physical properties, which can be described as the sum of local abiotic factors such as insolation (sunlight), climate, and geology, and biotic factors, which are other organisms that share its habitat. (wikipedia encyclopedia) Watershed:
Every stream, regardless of its size, has a watershed. Smaller watersheds are contained within larger watersheds. For example, the French Creek watershed in southeastern Pennsylvania is part of the Schuylkill watershed, which in turn is part of the Delaware River Watershed, which is part of the Delaware Bay watershed. No matter where you live, you live within a watershed. Just as you have a home and school address, you also have a watershed address. Get to know your watershed address. (Stroud Water Research Center. http://www.stroudcenter.org/lpn/more/watersheds.htm)
Animated Video of a watershed: Approx. 40 sec long Please give it a moment to load. (Note: video clip from the Conservation Technology Information Center website)
Macroinvertebrates:
Leaf Pack: Please read "Interview with a scientist Dr. Bernard Sweeney" for a complete understanding of the use of leaf packs in studing stream ecology. You will leave the Bryn Mawr College Website when you click on this link.
Aerial Map of Rhoads Pond at Bryn Mawr College
Aerial Map of Rhoads Pond by Don Barber Mapping LabCan you find where Rhoads Pond and Mill Creek are?Exercise #1. 1. Open a web browser and go to http://www.topozone.com/. 2. In the lower left corner, you'll see "Place Name Search." In this box, type "Bryn Mawr College" and select "PA" for state, then click "Search." 3. When the next page appears, you'll see a prompt at the top of the page telling you to click on the Place name link. Click on "Bryn Mawr College."
4. On the next page, you'll see an image of a map that is "zoomed in" on Bryn Mawr College. On the lefthand side, change the map size from "small" to "large" so you can see more of the area surrounding Bryn Mawr. 5. Locate Mill Creek. 6. Using the scale selection drop box ("View scale") and the arrows that point left, right, up, and down, trace the path of Mill Creek to the ocean. Consider these questions:
1. How far do you think an organism we find at Bryn Mawr College would go along this path? 2. What other bodies of water might it encounter along the way? 3. Would it pass landmarks that you recognize? 4. Where do you think it would end up? Exercise #2:
2. On top of the map of the United States, click "Satellite." 3. Again, search for Bryn Mawr College and click on the first entry. 4. See if you can repeat the activity above using the satelite images.
2. How do humans impact/interact with watersheds?
This mapping lab was developed by Kailin Friedman (06) a BMC Geology/ES undergradute student |
Ecosystem Ecology Watershed Macro-invertebrate Leaf Pack
Excersize:
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